Giuliana Rancic hasn’t given up on her quest to becoming a mother-of-two — but she only has one chance left.

The E! News host tells 'Life & Style' that she and her husband, Bill, still have one frozen embryo, and they’re hoping it will one day become a baby brother or sister for their two-year-old son, Duke.

“This could be our last shot,” Giuliana confides to the mag. “We’re keeping our fingers crossed that everything works out.”

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Giuliana with Duke.

It’s the latest twist in a years-long fertility struggle for the couple, whose son was born via a surrogate who then suffered a miscarriage while pregnant with the couple's second child in 2014.

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Giuliana and hubby Bill.

Whatever the outcome, the 40-year-old — who received major backlash last week after making an offensive comment on TV — says she’ll stay positive. Not only does she have a wonderful husband and toddler, but she’s also happily designing her own fashion line, G by Giuliana for HSN.

“If I don’t have another kid,” she says, “I have to remind myself that I’m very, very fortunate to have what I have.”